BRAIN STORM (215486)

  https://cordis.europa.eu/project/id/215486

  FP7 (2007-2013)

  On-chip simultaneous intracellular recording and stimulation of electrical and biochemical activities from hundreds of neurons

  Bio-ICT convergence (ICT-2007.8.3)

  sensors

  2008-01-01 Start Date (YY-MM-DD)

  2010-12-31 End Date (YY-MM-DD)

  € 4,241,967 Total Cost

  Description

In the BRAINSTORM project, we aim to develop an integrated system enabling on-chip intracellular recordings from a relatively large cultured neuronal network combined with chemical sensing and intracellular delivery of biologically active molecules. To achieve tight coupling with the cell membrane and enable efficient recordings, micronail-type probes will be integrated with a CMOS readout circuitry. These micronails are structured to operate either as intracellular microelectrodes or as intracellular micro-syringes allowing either chemical injection or to produce pressure or suction that will be used in additional to other procedures to penetrate the cell membrane. With appropriate bio-functionalization, the micro-nails inserted intracellularly will also serve as sensors for phosphorylation/ dephosphorylation reactions within living cells.<br/>The main technologies and methods to be developed in the BRAINSTORM project include:<br/>•\tBiocompatible and CMOS compatible micronails and micro-syringes with integrated readout electronics;<br/>•\tMicrofluidic system integrated with the CMOS-containing chip;<br/>•\tProcedures to insert both nail types through the plasma membrane, into the neuronal cytosole;<br/>•\tSurface chemistries allowing to achieve a Giga-Ohm (G) seal resistance between the disrupted plasma membrane and the nail;<br/>•\tHigh signal-to-noise ratio recordings of neuronal electrical activity with micronail probes;<br/>•\tOn-chip chemical delivery of chemicals;<br/>•\tOn-chip sensing of intracellular kinase activities.<br/>

  Complicit Organisations

1 Israeli organisation participates in BRAIN STORM.