NO-CANCER (256438)

  https://cordis.europa.eu/project/id/256438

  FP7 (2007-2013)

  Protein S-Nitrosylation in Inflammation and Cancer

  Marie Curie Action: "Reintegration Grants" (FP7-PEOPLE-2009-RG)

  proteomics  ·  lung cancer  ·  pathology

  2010-04-01 Start Date (YY-MM-DD)

  2014-03-31 End Date (YY-MM-DD)

  € 100,000 Total Cost

  Description

Chronic inflammation represents a major pathologic basis for many human cancers. Nitric oxide (NO) production is a hallmark of inflammation, and may play a significant role in inflammation-associated cancers. S-nitrosylation, the covalent attachment of an NO group to the thiol side chain of cysteine is a common mechanism for dynamic, post-translational regulation of most or all main classes of protein. Indeed, protein S-nitrosylation and denitrosylation have emerged as integral components of signal transduction pathways, and accumulating evidence suggests that deregulated S-nitrosylation contributes to a range of human pathologies. Yet, the involvement of protein S-nitrosylation/denitrosylation in inflammation-related cancer remains unclear. To elucidate the role of protein S-nitrosylation in inflammation-associated cancer we will employ models of lung cancer, where we will characterize the S-nitrosylated proteome, investigate denitrosylation mechanisms, and study the redox regulation of central mediators of inflammation. We focus on three areas of research: (1) advanced redox proteomics for the identification S-nitrosylated proteins in lung tumour cells and in lung macrophages; (2) characterization of the regulation by S-nitrosylation of key inflammatory mediators, particularly, the inflammasome; (3) investigation of mechanisms and consequences of protein denitrosylation in lung cancer cells.

  Complicit Organisations

1 Israeli organisation participates in NO-CANCER.