ESCRT HIGHRES (332720)

  https://cordis.europa.eu/project/id/332720

  FP7 (2007-2013)

  Employing quantitative high-resolution imaging to understand mechanistic principles of ESCRT-mediated membrane fission in dividing cells

  Marie-Curie Action: "Career Integration Grants" (FP7-PEOPLE-2012-CIG)

  proteins  ·  microscopy  ·  heterocyclic compounds

  2013-03-01 Start Date (YY-MM-DD)

  2017-02-28 End Date (YY-MM-DD)

  € 100,000 Total Cost

  Description

The ESCRT machinery is a multi-protein complex involved in membrane constriction and fission in a variety of processes in cells including receptor degradation, viral budding and cytokinesis. The mechanism by which ESCRT proteins drive membrane fission in cells is still largely unknown. Using super resolution fluorescence microscopy and quantitative live cells imaging I have recently mapped the spatiotemporal organization of ESCRTs in cytokinesis. The results identified a sequential recruitment of ESCRT proteins into a series of partially overlapping cortical ring structures and indicated the ESCRT machinery as the driving force for the membrane cleavage event required for cell separation. This work highlighted the advantage of using cytokinesis as a model system for studying ESCRT mediated membrane fission in a physiologically relevant context. In the proposed project I aim to elucidate how ESCRT function is regulated in cytokinesis in order to unravel the mechanism by which ESCRT proteins drive membrane fission in cells. Using super resolution imaging and quantitative live cell imaging of ESCRT proteins in genetically manipulated cells I will identify factors that regulate ESCRT function in cytokinesis and determine their effect on the spatiotemporal organization of ESCRTs in cytokinesis. Unraveling the regulatory basis for ESCRT function will underlie fundamental steps in the ESCRT pathway required for driving membrane fission in cytokinesis and will contribute critical information for understanding the mechanistic basis for ESCRT mediated membrane fission. The results of this work will have great implication to other ESCRT mediated cellular events including receptor degradation and viral budding.

  Complicit Organisations

1 Israeli organisation participates in ESCRT HIGHRES.